pcdna3 1 mycbioid vector (Addgene inc)
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Addgene inc
pcdna3 1 mycbioid vector
Pcdna3 1 Mycbioid Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+1+mycbioid+vector/pcDNA3%2E1+mycBioID+(Plasmid+%2335700)/pmc12070652-453-28-30
Average 94 stars, based on 112 article reviews
Pcdna3 1 Mycbioid Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+1+mycbioid+vector/pcDNA3%2E1+mycBioID+(Plasmid+%2335700)/pmc12070652-453-28-30
Average 94 stars, based on 112 article reviews
pcdna3 1 mycbioid vector - by Bioz Stars,
2026-10
94/100 stars
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Plasmid Preparation:Article Title: α-Synuclein A53T Binds to Transcriptional Adapter 2-Alpha and Blocks Histone H3 Acetylation Article Snippet: Donkey anti-mouse IgG H&L (Alexa Fluor 488) (Ab150105, Abcam, Cambridge, UK), donkey anti-rabbit IgG H&L (Alexa Fluor 594) (Ab150076, Abcam, Cambridge, UK), and biotin-SP-AffiniPure goat anti-rabbit IgG H+L (111-065-045, Jackson ImmunoResearch, Philadelphia, PA, USA) were used as secondary antibodies for the immunofluorescence and immunohistochemistry assays. .. The Article Title: NEMO is essential for directing the kinases IKKα and ATM to the sites of DNA damage Article Snippet: After being washed, the membranes were incubated with specific secondary horseradish peroxidase-linked antibodies from Dako and visualized using enhanced chemiluminescence reagent [Biological Industries] and autoradiography films [GE Healthcare]. .. BirA-IKKα plasmid encoding human IKKα protein sequence in the C-terminus of BirA (R118G) sequence was obtained from Protein Technologies unit form CRG by subcloning IKKα coding sequence into Article Title: The comprehensive interactomes of human adenosine RNA methyltransferases and demethylases reveal distinct functional and regulatory features Article Snippet: .. To prepare the common backbone for inducible expression of the modified version of BirA (R118G, BirA*), the BirA* sequence was amplified from Article Title: Cytoskeletal association of ATP citrate lyase controls the mechanodynamics of macropinocytosis. Article Snippet: Human CPα (CAPZA1, cloneID 3047937) and CPβ (CAPZB, clone ID 6715709) were purchased from Dharmacon and inserted into pcDNA3 (Invitrogen) with N-terminal MYC or FLAG tags. .. BirA-ACLY constructs were generated by inserting human ACLY into Article Title: Tropomodulins Control the Balance between Protrusive and Contractile Structures by Stabilizing Actin-Tropomyosin Filaments Article Snippet: .. To obtain a plasmid for expressing BirA fused to the N terminus of Tpm3.1, human Tpm3.1 cDNA was amplified and subcloned into the Article Title: Cytoskeletal association of ATP citrate lyase controls the mechanodynamics of macropinocytosis Article Snippet: Human CPα (CAPZA1, cloneID 3047937) and CPβ (CAPZB, clone ID 6715709) were purchased from Dharmacon and inserted into pcDNA3 (Invitrogen) with N-terminal MYC or FLAG tags. .. BirA-ACLY constructs were generated by inserting human ACLY into Sequencing:Article Title: NEMO is essential for directing the kinases IKKα and ATM to the sites of DNA damage Article Snippet: After being washed, the membranes were incubated with specific secondary horseradish peroxidase-linked antibodies from Dako and visualized using enhanced chemiluminescence reagent [Biological Industries] and autoradiography films [GE Healthcare]. .. BirA-IKKα plasmid encoding human IKKα protein sequence in the C-terminus of BirA (R118G) sequence was obtained from Protein Technologies unit form CRG by subcloning IKKα coding sequence into Article Title: The comprehensive interactomes of human adenosine RNA methyltransferases and demethylases reveal distinct functional and regulatory features Article Snippet: .. To prepare the common backbone for inducible expression of the modified version of BirA (R118G, BirA*), the BirA* sequence was amplified from Subcloning:Article Title: NEMO is essential for directing the kinases IKKα and ATM to the sites of DNA damage Article Snippet: After being washed, the membranes were incubated with specific secondary horseradish peroxidase-linked antibodies from Dako and visualized using enhanced chemiluminescence reagent [Biological Industries] and autoradiography films [GE Healthcare]. .. BirA-IKKα plasmid encoding human IKKα protein sequence in the C-terminus of BirA (R118G) sequence was obtained from Protein Technologies unit form CRG by subcloning IKKα coding sequence into Article Title: Identification of the interacting partners of a lysosomal membrane protein in living cells by BioID technique Article Snippet: .. Timing: 4 weeks As an example, we constructed the cMyc-BirA-SLC15A4 (hereafter BirA-SLC15A4) expression vector by subcloning full-length human SLC15A4 cDNA into the Expressing:Article Title: The comprehensive interactomes of human adenosine RNA methyltransferases and demethylases reveal distinct functional and regulatory features Article Snippet: .. To prepare the common backbone for inducible expression of the modified version of BirA (R118G, BirA*), the BirA* sequence was amplified from Article Title: Tropomodulins Control the Balance between Protrusive and Contractile Structures by Stabilizing Actin-Tropomyosin Filaments Article Snippet: .. To obtain a plasmid for expressing BirA fused to the N terminus of Tpm3.1, human Tpm3.1 cDNA was amplified and subcloned into the Article Title: Identification of the interacting partners of a lysosomal membrane protein in living cells by BioID technique Article Snippet: .. Timing: 4 weeks As an example, we constructed the cMyc-BirA-SLC15A4 (hereafter BirA-SLC15A4) expression vector by subcloning full-length human SLC15A4 cDNA into the Modification:Article Title: The comprehensive interactomes of human adenosine RNA methyltransferases and demethylases reveal distinct functional and regulatory features Article Snippet: .. To prepare the common backbone for inducible expression of the modified version of BirA (R118G, BirA*), the BirA* sequence was amplified from Amplification:Article Title: The comprehensive interactomes of human adenosine RNA methyltransferases and demethylases reveal distinct functional and regulatory features Article Snippet: .. To prepare the common backbone for inducible expression of the modified version of BirA (R118G, BirA*), the BirA* sequence was amplified from Article Title: Tropomodulins Control the Balance between Protrusive and Contractile Structures by Stabilizing Actin-Tropomyosin Filaments Article Snippet: .. To obtain a plasmid for expressing BirA fused to the N terminus of Tpm3.1, human Tpm3.1 cDNA was amplified and subcloned into the Construct:Article Title: Cytoskeletal association of ATP citrate lyase controls the mechanodynamics of macropinocytosis. Article Snippet: Human CPα (CAPZA1, cloneID 3047937) and CPβ (CAPZB, clone ID 6715709) were purchased from Dharmacon and inserted into pcDNA3 (Invitrogen) with N-terminal MYC or FLAG tags. .. BirA-ACLY constructs were generated by inserting human ACLY into Article Title: Cytoskeletal association of ATP citrate lyase controls the mechanodynamics of macropinocytosis Article Snippet: Human CPα (CAPZA1, cloneID 3047937) and CPβ (CAPZB, clone ID 6715709) were purchased from Dharmacon and inserted into pcDNA3 (Invitrogen) with N-terminal MYC or FLAG tags. .. BirA-ACLY constructs were generated by inserting human ACLY into Article Title: Identification of the interacting partners of a lysosomal membrane protein in living cells by BioID technique Article Snippet: .. Timing: 4 weeks As an example, we constructed the cMyc-BirA-SLC15A4 (hereafter BirA-SLC15A4) expression vector by subcloning full-length human SLC15A4 cDNA into the Generated:Article Title: Cytoskeletal association of ATP citrate lyase controls the mechanodynamics of macropinocytosis. Article Snippet: Human CPα (CAPZA1, cloneID 3047937) and CPβ (CAPZB, clone ID 6715709) were purchased from Dharmacon and inserted into pcDNA3 (Invitrogen) with N-terminal MYC or FLAG tags. .. BirA-ACLY constructs were generated by inserting human ACLY into Article Title: Cytoskeletal association of ATP citrate lyase controls the mechanodynamics of macropinocytosis Article Snippet: Human CPα (CAPZA1, cloneID 3047937) and CPβ (CAPZB, clone ID 6715709) were purchased from Dharmacon and inserted into pcDNA3 (Invitrogen) with N-terminal MYC or FLAG tags. .. BirA-ACLY constructs were generated by inserting human ACLY into |